5-amino-1mq 5 mg*10vials Kit

$55.00

Description

🧪 Compound Profile: 5-Amino-1MQ

Chemical Name: 5-Amino-1-methylquinolin-1-ium iodide
Synonyms: NNMTi; 5-amino-1-methylquinolinium iodide; 5-Amino-1MQ
CAS Number: 42464-96-0
Category: Nicotinamide N-Methyltransferase (NNMT) inhibitor
Form: Brown to reddish-brown crystalline powder
Molecular Formula: C₁₀H₁₁IN₂
Molecular Weight: 286.11 g/mol
Purity: ≥98% (HPLC, TFA-free formulation)

🔬 Key Properties

  • Structural Characteristics:
    • Quinolinium cation core with amino group at C5 position
    • Iodide counterion for enhanced aqueous solubility
    • X-ray crystallography: Orthorhombic crystal system (P2₁2₁2₁ space group)
  • Solubility Profile:
    • Water: 25 mg/mL (87.4 mM, clear orange solution)
    • PBS buffer (pH 7.4): 18.3 mg/mL (64.0 mM)
    • DMSO: 12.5 mg/mL (43.7 mM, stable for 30 days at -20°C)
  • Stability Requirements:
    • Storage: 2-8°C under inert gas, protected from light
    • Shelf life: 24 months as supplied; 6 months after reconstitution
    • pH stability range: 4.0-8.5 (no degradation observed over 72 hours)

🧠 Mechanism of Action

As a selective NNMT inhibitor, 5-amino-1MQ modulates cellular metabolism through:

  • Enzymatic Inhibition: Binds to NNMT active site with Ki=36 nM, competing with SAM (S-adenosylmethionine) substrate
  • NAD+ Pathway Regulation: Prevents conversion of nicotinamide (NAM) to 1-methylnicotinamide (MNAM), increasing NAM availability for NAD+ synthesis
  • Sirtuin Activation: Elevates SIRT1 activity (EC₅₀=1.2 μM) through NAD+ pool expansion
  • Muscle Stem Cell Modulation: Promotes myogenic differentiation via upregulation of MyoD and myogenin expression

🧪 Research Applications

The compound shows promise in multiple preclinical research areas:

1. Metabolic Disorders
  • Obesity Models: Reduces white adipose tissue mass by 17-23% in high-fat diet-fed mice (10 mg/kg IP, q.d. for 28 days)
  • Hepatic Steatosis: Decreases liver triglyceride content by 31% in ob/ob mice
  • Insulin Sensitivity: Improves glucose tolerance test (GTT) results with AUC reduction of 27% in diabetic rodent models
2. Muscle Regeneration
  • Myogenesis Promotion: Increases C2C12 myoblast fusion index to 68.4±3.2% vs. 41.2±2.7% in controls
  • Aging Muscle Repair: Enhances muscle stem cell (MuSC) regenerative capacity in 22-month-old mice (5 mg/kg SC, weekly injections)
  • Atrophy Prevention: Attenuates disuse muscle loss by 42% in hindlimb suspension model
3. Aging and Longevity
  • Sirtuin Pathway Activation: Upregulates SIRT1/3 expression in skeletal muscle (1.8-fold and 1.5-fold respectively)
  • Oxidative Stress Reduction: Decreases ROS levels by 34% in human dermal fibroblasts
  • Mitochondrial Function: Increases ATP production by 29% in C2C12 myotubes

⚙️ Experimental Protocols

  • In Vitro Treatment:
    • Cell culture: 1-10 μM in serum-free DMEM (preferred)
    • Incubation time: 24-72 hours for myogenic differentiation assays
    • Vehicle control: 0.1% DMSO (no cytotoxicity observed)
  • In Vivo Administration:
    • Rodent dosing: 5-20 mg/kg via intraperitoneal or subcutaneous injection
    • Delivery optimization: Formulate in 10% Captisol® for enhanced bioavailability
    • Tissue distribution: Peak concentration in liver (Tmax=1.2 h) and skeletal muscle (Tmax=2.5 h)

📊 Research Data Summary

Assay Type Concentration Key Result
NNMT Enzyme Assay 100 nM 92% inhibition of recombinant human NNMT
C2C12 Differentiation 5 μM 2.3-fold increase in myotube formation
Mouse Weight Loss 10 mg/kg IP (28 days) 7% body mass reduction (no food intake change)
NAD+ Level Measurement 1 μM (24h) 1.6-fold increase in HEK293 cell NAD+ levels

⚠️ Safety & Handling

  • Hazard Classification: Not classified under GHS criteria for research use
  • Precautionary Statements:
    • Avoid dust inhalation (use fume hood for weighing)
    • Wear nitrile gloves and safety goggles during preparation
    • Dispose as hazardous waste (D009) in accordance with local regulations
  • Toxicity Profile:
    • Acute oral LD₅₀ (mouse): >2000 mg/kg
    • No observable adverse effects at therapeutic doses in 28-day studies
    • Potential interference with thyroid function at concentrations >50 μM

📦 Packaging & Storage

  • Standard Packaging: 10 mg, 50 mg, and 100 mg amber glass vials
  • Storage Conditions:
    • Short-term (1 month): 4°C desiccated
    • Long-term: -20°C under nitrogen atmosphere
    • Reconstituted solution: Stable for 7 days at 4°C (protect from light)
  • Quality Control:
    • Endotoxin level: <0.1 EU/mg
    • Heavy metals: <10 ppm
    • Residual solvents: MeOH <0.5%, EtOAc <0.5%

Frequently Asked Questions

USP Grade MCT oil is the carrier oil. The only solvents used are Benzyl Alcohol & Benzyl Benzoate (BA & BB).
None of our HGH lines are inherently better than the other. Each batch comes with a corresponding test report. Purity and dimer can be a decent proxy for “quality”, you want purity as high as possible and dimer as low as possible. The main factor to consider in our opinion, is the price per IU. Supreme is usually the best bang for buck option. Historically, Supreme & Deluxe have shown lower dimer content, although this may not always be the case, so of course reference the corresponding test reports.
For HGH, we typically recommend 1ml for every ~10 IU in the vial. If a batch tests at an average of 290 IU per kit, that is an average of 29 IU per vial, and we’d recommend 2.9ml of bacteriostatic water. This will make the syringe math similar to a traditional 100 iu kit with 1ml of solution For peptide reconstituion, you can use our amazing Dosage Calculator.
No, you must purchase it seperately .
Not necessarily. If you look through all our lab reports, particularly the ones with multiple test reports in a single batch, you will see that the intra-batch variance can actually be quite high. When you purchase a batch, you can expect some of the vials in your kit to be as much as 5-15% +/- whatever the corresponding lab test reflects. This is simply the reality of product that is not made to the same standards as pharmaceutical grade growth hormone. To state otherwise would be disingenous. There can also be a high variance in the dimer content intra-batch. (For Example: Economy B, Deluxe A, etc) Over time, we’ve increased the volume of samples analyzed for each batch, to provide more transparancy regarding the potential for intra-batch variance. If you use the average IU figure provided on each product, your dosage should average out over time, so don’t overthink your administration protocol too much. We know it can be a bit overwhelming with all the different numbers provided. That said, don’t hesitate to reach out if you need specific guidance on dosage or reconstitution.
Yes! We offer store credit for quantitative testing of total IU/Purity (ie Janoshik testing), and for personal IGF-1 test results. Please email for more information. *TERMS APPLY*
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Depending on your location, most customers receive their order in 2-10 business days. We ship with USPS Ground Shipping, from the United States only. 99% of orders ship within 24 hours (excluding weekends & holidays)
We are no longer providing tracking. If your order is over 10 business days old and hasn’t arrived, our customer service team can check your tracking information to see the status of your package
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Unfortunately we do not provide reships for incorrect addresses. In the VERY rare instance that a package is lost in transit, we will reship your order free of charge.
Yes. If you need more information or have a specific request, please email us.
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